Abstract
Lasiodiplodan is a β-glucan polymer with different interesting characteristics, including therapeutic properties. It is an extracellular product, which is produced by the filamentous fungus Lasiodiplodia theobromae, using glucose as a substrate. In the present work, the production of lasiodiplodan was studied by the utilization of sugarcane straw as a low-cost carbon source. Glucose-rich sugarcane straw hydrolysate was obtained by a sequential pretreatment with dilute nitric acid (1% v/v) and sodium hydroxide (1% w/v), followed by enzymatic hydrolysis. The fermentation process was conducted by the cultivation of the strain Lasiodiplodia theobromae CCT3966 in sugarcane straw hydrolysate in a shake flask at 28 °C for 114 h. It was found that hydrolysate obtained after enzymatic hydrolysis contained 47.10 gL−1 of glucose. Fermentation experiments of lasiodiplodan synthesis showed that the peak yield and productivity of 0.054 gg−1 glucose consumed and 0.016 gL−1 h−1, respectively, were obtained at 72 h fermentation time. Fungal growth, glucose consumption, and lasiodiplodan production from sugarcane straw hydrolysate presented a similar pattern to kinetic models. The study on the chemical structure of lasiodiplodan produced showed it had a β-glucan construction. The current study revealed that sugarcane straw is a promising substrate for the production of lasiodiplodan.
Highlights
IntroductionThe filamentous fungus Lasiodiplodia theobromae has received a growing interest by researchers for the biosynthesis of an extracellular β-glucan polymer, namely lasiodiplodan, since this fungus has a high potential for industrial production level [1,4]
The chemical composition profile of sugarcane straw pretreated with dilute nitric acid revealed that the hemicellulosic content in untreated sugarcane straw reached 3.53% after acid pretreatment
This research showed the feasibility of lasiodiplodan production using the fermentable glucose obtained from sugarcane straw pretreated by HNO3 (1% v/v) and NaOH (1% w/v)
Summary
The filamentous fungus Lasiodiplodia theobromae has received a growing interest by researchers for the biosynthesis of an extracellular β-glucan polymer, namely lasiodiplodan, since this fungus has a high potential for industrial production level [1,4] In this regard, the tremendous biological characteristics of lasiodiplodan such as anti-proliferative effect on breast cancer cells, hypoglycemic function, and antioxidant activities have made it an attractive biopolymer for commercial applications [5,6]. Current efforts for microbial synthesis of lasiodiplodan are based on the utilization of commercial sugars, glucose [4,5,8,9,10] These sugars are mostly obtained from unsustainable carbon sources such as food-derived carbohydrates, which strongly compete with food chains [11,12].
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