Abstract
BackgroundFusarium wilt of banana is one of the most destructive diseases in banana-growing regions worldwide. Soil-borne diseases and soil microbial communities are closely related. The screening of antagonistic bacteria from soil microorganisms in areas with Fusarium wilt of banana is of great practical significance for controlling this disease.ResultsA strain designated FS-4 was isolated from healthy banana rhizosphere soil in an area affected by Fusarium wilt. This strain exhibited a significant antagonistic effect on the pathogen. Pot experiments revealed that the fermentation broth of strain FS-4 not only decreased the incidence of banana Fusarium wilt, but also promoted the growth of banana seedlings. The strain was identified as Streptomyces ma. by its morphological, physiological, and biochemical characteristics and 16S rRNA gene sequence analysis. The culture and fermentation conditions for this strain were optimized by single-factor and response surface experiments. The optimum culture conditions for Streptomyces ma. FS-4 were as follows: peptone 0.5%, saccharose 2.4, 0.05% K2HPO4, 0.05% MgCl2, and 0.05% NaCl at an initial pH of 7.0; 180 g at 28 °C; and inoculation size of 6% for 62 h. The diameter of bacteriostasis circle for Bacillus subtilis reached 26.7 mm.ConclusionStreptomyces ma. FS-4 is an important microbial resource as a biological agent for the control of plant pathogenic fungi and can be used to promote banana growth.
Highlights
Fusarium wilt of banana is one of the most destructive diseases in banana-growing regions worldwide
Mingma et al (2014) isolated actinomycetes from legumes fermentation liquid and identified phenylacetic acid as the active substance that inhibited the pathogen of soybean glycine Xanthomonas [14]. These results provided new ideas and approaches for the biological control of Fusarium wilt of banana
Pathogenic fungi strains includes Fusarium oxysporum f. sp. cubense Race1 (FOC.1), F. oxysporum f. sp. cubense Race 4 (FOC.4), Curvulatia fallaxis (CFO), Colletotrichum gloeosporides (CG), and Alternaria tenuissima Maa (MAA), Escherichia coli (E. coli), Bacillus subtilis (B. subtilis), and Staphylococcus aureus (S. aureus)were provided by the Institute of Environment and Plant Protection
Summary
Fusarium wilt of banana is one of the most destructive diseases in banana-growing regions worldwide. The screening of antagonistic bacteria from soil microorganisms in areas with Fusarium wilt of banana is of great practical significance for controlling this disease. Among the antibiotics produced from actinomycetes, only extremely small doses will exert a significant effect and can effectively prevent and control a variety of infectious diseases in humans and livestock [16, 18, 19]. Researchers have screened different actinomycete strains from soils and plants. These strains can control turfgrass root rot [20], rice blast [21], rice leaf blight [18], and tomato gray mold [22] with good results.
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