Abstract
BackgroundPanax notoginseng is one of the most valuable traditional Chinese medicines. Polysaccharides in P. notoginseng has been shown to significantly reduce the incidence of human diseases. However the application of fermentation technology in Panax notoginseng is not common, and the mechanism of action of P. notoginseng polysaccharides produced by fermentation is still unclear. The specific biological mechanisms of fermented P. notoginseng polysaccharides (FPNP) suppresses H2O2-induced apoptosis in human dermal fibroblast (HDF) and the underlying mechanism are not well understood.MethodsIn this study, the effects of water extracted and fermentation on concentration of polysaccharides in P. notoginseng extracts were analyzed. After the H2O2-induced HDF model of oxidative damage was established, and then discussed by the expression of cell markers, including ROS, MDA, SOD, CAT, GSH-Px and MMP-1, COL-I, ELN, which were detected by related ELISA kits. The expression of TGF-β/Smad pathway markers were tested by qRT-PCR to determine whether FPNP exerted antioxidant activity through TGF-β signaling in HDF cells.ResultsThe polysaccharide content of Panax notoginseng increased after Saccharomyces cerevisiae CGMCC 17452 fermentation. In the FPNP treatment group, ROS and MDA contents were decreased, reversed the down-regulation of the antioxidant activity and expression of antioxidant enzyme (CAT, GSH-Px and SOD) induced by H2O2. Furthermore, the up-regulation in expression of TGF-β, Smad2/3 and the down-regulation in the expression of Smad7 in FPNP treated groups revealed that FPNP can inhibit H2O2-induced collagen and elastin injury by activating TGF-β/Smad signaling pathway.ConclusionIt was shown that FPNP could inhibit the damage of collagen and elastin induced by H2O2 by activating the TGF-β/Smad signaling pathway, thereby protecting against the oxidative damage induced by hydrogen peroxide. FPNP may be an effective attenuating healing agent that protects the skin from oxidative stress and wrinkles.
Highlights
Panax notoginseng is one of the most valuable traditional Chinese medicines
It was shown that fermented P. notoginseng polysaccharides (FPNP) could inhibit the damage of collagen and elastin induced by H2O2 by activating the Transforming growth factor-β (TGF-β)/Smad signaling pathway, thereby protecting against the oxidative damage induced by hydrogen peroxide
Fermentation of P. notoginseng root (PNR) by Saccharomyces cerevisiae CGMCC 17452 The concentrations of polysaccharides, ginsenosides and flavonoids were measured in water-extracted PNR (WPNR) and fermented PNR (FPNR) by S. cerevisiae CGMCC 17452
Summary
Panax notoginseng is one of the most valuable traditional Chinese medicines. Polysaccharides in P. notoginseng has been shown to significantly reduce the incidence of human diseases. Relevant studies have shown that P. notoginseng has many pharmacological effects, and it has been used in a large proportion of clinical treatment of cardiovascular diseases [4], cerebrovascular [5], blood, nervous and immune systems [6], as well as wound healing [7,8,9,10]. It is added as the main ingredient in topical Chinese patent medicines, such as Yunnan Baiyao and Pianzaihuang, for hemostasis, antiulcer and protection against premature aging. By adding concentrated P. notoginseng fermented extracts, these prescriptions can promote the synthesis of collagen to reduce fine lines and wrinkles and effectively repair damage caused by sunlight or the external environment [12]
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