Abstract
In this study, amperometric enzyme electrodes based on polymer film for determination phenolic compounds were prepared. For this purpose platin electrodes were modified by electropolymerization using asetonitril-water mixture containing o-phenylenediamine (o-PD) or o-phenylenediamine C and -0.20 V (vs. Ag/AgCl) respectively. The linear working range of 1.96×10-6-3.5×10-5 M. Response time, reusability, life time, and amperometric responses to other phenolic compounds were also investigated.and 3-methylthiophene (3MT). Polyphenol oxidase was immobilized onto Pt/PoPD and Pt/PoPD-3MT electrodes and amperomeric responses to pyrocatechol were investigated. Pt/PoPD-3MT/POx electrode that obtained higher sensitivity was used for determination of working conditions and performance factors. Optimum type of buffer, buffer concentration, pH, temperature and working potential were determinated as phos
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