Abstract
The article contains new information on the current problem of the influence of endo- and exopathogenic factors on the structure of the digestive system. The purpose of the study was to establish the structural features of the interlobular bile ducts under the conditions of six weeks of exposure to nalbuphine in the experiment. The study was based on 30 white laboratory rats of male reproductive age. Histological sections of the liver were stained with hematoxylin and eosin according to conventional methods. The «Aver Media» computer system was used to photograph the micropreparations. The computer program ImageJ was used to measure the diameter of the lumen and the wall thickness of the interlobular bile duct. «Excel» and «Statistica» 6.0 software were used for statistical processing of the obtained digital data. The results of the study show that against the background of nalbuphine administration for six weeks there are morphological changes in the interlobular bile ducts of experimental animals, manifested by destructuring of the interlobular bile ducts, loss of their proper shape, expansion and unevenness of their lumens, deformation and thickening of the interstitial walls in 2-3 times, disorganization of cholangiocytes, thinning of the cell layer due to detachment of cholangiocytes, polymorphism of their nuclei, stratification, fragmentation of the lamina propria of the interlobular bile ducts, periductal edema, dilatation of interlobular portal tracts, "varicose" veins of the interlobular portal tracts, pronounced smooth muscle hyperplasia of the interlobular arteries, the presence of perivascular lymphocytic infiltrates. The data of the study can be used in practical medicine in the diagnosis and treatment of pathology of the bile ducts due to long-term use of opioids.
Highlights
Pathology of the hepatobiliary system is a problem relevant to modern medicine [10, 16, 22]. 388 people per 10 thousand population are registered with diseases of the biliary system, which is up to 50 % of patients with pathology of the digestive system
The wall of the interlobular bile ducts is lined with a single layer of cubic fringed epitheliocytes cholangiocytes, which are adjacent to its own plate, formed by a thin layer of connective tissue (Fig. 1)
After 4 weeks of experimental exposure to nalbuphine, noted signs of inflammation of the interlobular bile ducts, deformation and expansion of the lumen of the interlobular bile ducts, their diameter was 11.13±0.91 μm, and their wall thickening to 5.474±0.361 μm was noted against the background of liver particle destruction
Summary
Pathology of the hepatobiliary system is a problem relevant to modern medicine [10, 16, 22]. 388 people per 10 thousand population are registered with diseases of the biliary system, which is up to 50 % of patients with pathology of the digestive system. 388 people per 10 thousand population are registered with diseases of the biliary system, which is up to 50 % of patients with pathology of the digestive system. The negative effect of the use of drugs today creates a serious medical and social, and economic problem. It has been established that in cholestatic liver diseases the concentration of endogenous opioid peptides increases in blood plasma, which indicates a close functional relationship between the hepatobiliary and opioid systems and the role of the latter in the development of pathology [15]. The rapid progressive destruction of intrahepatic bile ducts associated with the use of narcotic agents [17], the effect of the opioid ketamine on the bile ducts [1, 24]. Modeling of a pathology on experimental animals allows a deeper study of morphological and functional changes in the body [5, 27]
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