Abstract
The improvement of the pharmacological strategy of non-alcoholic fatty liver disease is based on the study of the effect of pharmaceutical preparations on the structure and function of the liver. The pathogenesis of steatohepatitis is complex and multifactorial, mainly involving genetic, metabolic and environmental factors. The purpose of the study was to characterize the structural and functional parameters of the liver when using the biologically active compound Angiolin for the correction of experimental steatohepatitis. An experimental study was performed on 110 sexually mature white male rats weighing 180-220 grams, which were kept on a standard diet of vivarium. All animals were divided into two groups: control (30 intact animals) and experimental (80 animals). A model of non-alcoholic steatohepatitis was created for all animals of the experimental group. They were kept on a hypercaloric diet with a high fat and high cholesterol content for 8 weeks. After that, part of the animals (10 rats) was withdrawn from the experiment by intrapleural administration of sodium thiopental (50 mg/kg) and the necessary biochemical and morphological studies were performed. Part of the animals (30 rats) was continued to be kept on a high-fat diet for 4 weeks and the biologically active compound Angiolin was administered (20 rats), and Rings-Locke solutions were administered to 10 rats. After the creation of the model, the other animals of the experimental group (40 rats) were transferred to a full-fledged standard semi-synthetic starch-casein diet, and the biologically active compound Angiolin was administered for 20 rats and Ringer-Locke solution for another 20 rats for 4 weeks. Macroscopic evaluation and description of the liver of animals was carried out after withdrawal under thiopental anesthesia. Statistical analysis of the results was carried out using the program “STATISTICA 8” using parametric and non-parametric methods for assessing the results. It was found that the use of the biologically active compound Angiolin once a day for 30 days can reduce cytolysis syndrome (reduce biochemical parameters such as ALT, AST, gamma-glutamyl transpeptidase), reduce cholestasis syndrome (decrease in alkaline phosphatase level), and normalize liver function, improves the morphological state of hepatocytes, which indicates the normalization of the structural and functional state of the liver.
Highlights
In recent years, the global burden of obesity and diabetes has led to a parallel increase in other metabolic complications, such as non-alcoholic fatty liver disease (NAFLD)
Ukrainian scientists have established that the course of nonalcoholic steatohepatitis with secondary arterial hypertension is characterized by higher histological activity, which leads to the progression of fibrotic changes [11]
The use of high-fat diet (HFD) for 60 days resulted in an increase in body weight of 1.66 times, and after 90 days - of 1.87 times, with the body mass index (BMI) increasing by 1.32 and 1.39 times, respectively
Summary
The global burden of obesity and diabetes has led to a parallel increase in other metabolic complications, such as non-alcoholic fatty liver disease (NAFLD). NAFLD is the most common type of chronic liver injury in many countries [8, 17]. NAFLD includes a spectrum of syndromes ranging from simple steatosis, nonalcoholic steatohepatitis to fibrosis, cirrhosis and hepatocellular carcinoma [27]. Over the last 15 years, NAFLD has grown dramatically in parallel with two major epidemics worldwide - obesity and type 2 diabetes [9]. The World Gastroenterology Organization (WGO) has recently published a number of comprehensive guidelines for the evaluation and treatment of NAFLD [13], with an emphasis on the distinction between simple steatosis and non-alcoholic steatohepatitis (NASH). Ukrainian scientists have established that the course of nonalcoholic steatohepatitis with secondary arterial hypertension is characterized by higher histological activity, which leads to the progression of fibrotic changes [11]
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