Effect of angiolin on the functional state of the liver in experimental steatohepatitis

Abstract

For a real impact on the functional state of the liver in steatohepatitis, the appointment of metabolic correctors is suggested. The aim of the study is to evaluate the effect of the biologically active compound angiolin on markers of cytolysis and liver function in experimental nonalcoholic steatohepatitis. Experimental studies were performed on 110 white, non-linear, mature male rats weighing 180–200 g at the start of the experiment. A model of non-alcoholic steatohepatitis was created, rats were maintained for 8 weeks on a high-calorie, high-cholesterol diet. After creating the model some of the animals were transferred to a standard semi-synthetic starch-casein diet and administered for 4 weeks the biologically active compound Angiolin. The activity of enzyme markers of cytolysis — alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyltranspeptidase (GGT) in serum was determined by standard methods. Liver biosynthetic function was assessed by the level of total protein and albumin. Statistical analysis of the obtained results was carried out with the use of the license program “STATISTICA 8” by Statsoft. After creating a model of non-alcoholic fatty liver disease in rats, an increase in body mass index occurred, high activity of ALT, AST, GGT was manifested and the concentration of total protein and albumin in the blood serum decreased compared to intact animals. An inverse correlation of mean strength was found between body mass index and total protein concentration as well as serum albumin. A strong inverse correlation between the value of ALT activity and the concentration of total protein and albumin in blood serum was established. The use of angiolin contributed to an increase in the concentration of total protein and albumin in the blood serum, an increase in the albumin / globulin coefficient, and a decrease in cytolysis. It was concluded that the biologically active compound angiolin positively affects the parameters of cytolysis and protein synthesis of the liver.