Features of oral microbiota composition in patients with oral mucosal disease associated with classical and mediated acid-related gastrointestinal disorders

Abstract

Relevance. The authors established that classical and mediated acid-related gastrointestinal disorders affect the change in microbiota and the development of mucosal disease.Purpose. The study aimed to investigate the characteristics of oral microbiota in patients with mucosal disease associated with classical and mediated acid-related gastrointestinal disorders.Materials and methods. The study included 58 patients with the oral mucosal disease associated with chronic gastritis and duodenitis, pancreatitis secondary to gallstones associated with stomach hypersecretion. The comparison group consisted of 25 subjects without oral mucosal disease, with previously diagnosed acid-related gastrointestinal disorders and eradicated Helicobacter pylori as of the clinical examination time.Results. The study detected a pH shift towards the acidic end of the scale in the oral fluid samples of subjects with oral aphthous ulcers compared to the group without oral mucosal disease (comparison group) (p < 0.001). The composition ratio of the studied microbiota from the surface of the oral aphthous ulcers in the main groups showed an increase in the number of Candida spp. by 1.7 and 3.2 times (p > 0.2), Enterobacteriaceae spp. – 1.7 and 2.6 times, (p > 0.2), Actinobacillus spp. – 1.4 and 2.0 times (p > 0.2), Staphylococcus spp. – 1.3 and 1.5 times (p > 0.2), Enterococcus spp. – 2.6 and 3.5 times (p > 0.2), and a decrease in Neisseria spp. by 1.9 and 3.1 times (p > 0.2). The studied microbiota of main group II (PSG associated with SH) demonstrated a significant increase in the above species, p < 0.05, and a significant decrease in Neisseria spp., at p<0.05.Conclusion. The studied aphthous ulcer surface microbiota, obtained from subjects with pancreatitis secondary to gallstones associated with stomach hypersecretion, revealed a significant overrepresentation of Gram +, Gram– facultatively anaerobic and opportunistic microorganisms contributing to the aggravation of the oral mucosal disease clinical features.