Features of eNOS and HTR2A genetic polymorphism in the implementation of the productioninduced cardiovascular pathology in workers at the underground mining enterprise

Abstract

Introduction. Identification of markers of genetic variability that determine the formation of professionally caused diseases of the cardiovascular system will allow to identify potential risk groups among workers and optimize the program of preventive and diagnostic measures.The aim of the study was to analyze the features of polymorphism of eNOS and HTR2A genes and regulatory indicators in workers at the mine mining enterprise with diseases of the cardiovascular system.Materials and methods. The men working at the enterprise on mine extraction of ore minerals, with pathology of cardiovascular system are examined. Real-time polymerase chain reaction and allelic discrimination were used to identify genotypes. Regulatory markers nitric oxide and serotonin were determined by enzyme immunoassay.Results. In the observation group, an increase in the frequency of polymorphic variants of eNOS G894T genotype GG 1.4 times and allele G 1.2 times, significantly associated with the development of cardiovascular disease (OR=3.16; Cl 95%=1.14– 8.76), as well as the presence of excessive frequency of minor allele G gene HTR2A (rs7997012) relative to the comparison group (1.8 times) due to mutant homozygous genotype GG (2.7 times) (OR=2.45; CL 95%=1.03–5.87), acting as risk FACTORS for cardiovascular disorders in the group of miners. At the same time, polymorphism of eNOS and HTR2A gene variants was combined with an imbalance in the level of CCC regulatory markers — nitric oxide and serotonin.Conclusions. Genetic variations of the eNOS g894t and HTR2A (rs7997012) genes can be recommended as sensitivity markers in monitoring and identification of risk groups among workers in underground mining.