Features of changes in prooxidant-antioxidant balance of tissues during activation of seed germination

Abstract

Aim of the research: to identify changes in the value of indicators of the state of the prooxidant-antioxidant system (PAS) in seed tissues at rest and the initiation of its germination processes. The subject of the research is the role of individual components of the PAS in ensuring the activation of seeds before germination. Methodology. Quantitative determination of indicators of the state of PAS was performed on tissue samples of seeds of the following plants: Glycine max L., Helianthus annuus L., Fagopyrum esculentum L., Linum usitatissimum L., Sinapis alba L., Chenopodium quinoa L., Panicum miliaceum L., Oryza sativa L., Avena sativa L., Zea mays L., Hordeum vulgare L., Triticum durum Desf. The concentration of superoxide anion radical (•O2-), TBA-active products, cytochrome oxidase activity, superoxide dismutase activity, catalase, the concentration of ascorbic acid, glutathione was determined. The results of the research show that for the tissues of seeds of experimental Magnoliopsida plants at rest, both links of PAS are more powerful than in Liliopsida, the level of free radical peroxidation (FRPO) is lower, which is achieved by both enzymatic and low molecular weight antioxidants (AO). Germination activation enhances both links of PAS in all experimental groups of plants, however, in Magnoliopsida, we observe the stronger generation of •O2-, and the predominance of protection by enzymatic AO, and in Liliopsida - low molecular weight.