Feasible Preparation of Naphthalimide-Functionalized Zeolitic Imidazolate Framework-8 for Control, and Fluorescence Detection of Virus: Optimization of Model Study.

Abstract

The onset of the coronavirus has highlighted the critical need to swift diagnostic techniques to identify the virus. Metal Organic Frameworks (MOFs) have attracted considerable attention in the field of sensing due to the remarkable characteristics. The present research examines the microwave preparation of naphthalimide-functionalized zeolitic imidazolate framework-8 (NI/ZIF-8) to the detection of coronavirus as a probe. The microwave synthesis approach facilitates an environmentally sustainable, and effective production of NI/ZIF-8. Characterization techniques have validated the material's high crystallinity and thermal behaviour. In order to enhance the performance of the probe, a 23 factorial model is implemented. This model facilitates the influences of control parameters, including NI/ZIF-8 dose, solution temperature, and buffer pH, on quenching response. The findings indicate the 27°C, pH 8.2, and a NI/ZIF-8 dose of 0.6mg/mL yields the maximum quenching response (83.21%). Additionally, it has been determined that photo electron transfer plays a significant role within the quenching process. The sensor demonstrated remarkable sensitivity to the COVID-19 RNA, achieving an exceptionally detection limit of 5.45 pM and a quick sense (20min), alongside a high detection selectivity of RNA. This project underscores the applicability of NI/ZIF-8 as an effective and eco-friendly sustainable substance for the rapid and accurate detection of RNA of COVID-19.