Abstract

This paper focuses on exploring new methods for preparing platelet-rich plasma (PRP) to improve its clinical application and popularization. As a natural growth factor, PRP has significant advantages in promoting tissue repair and alleviating inflammation, but limitations in preparation methods hinder its widespread use. The study uses clinical medical syringes as the centrifuge container for PRP preparation. Without disrupting the stratification of the blood, the method employs a pushing technique to move the density gradient centrifuged blood through a medical three-way valve, separating the different blood components to obtain the target PRP layer. This method is simple to operate, has a higher recovery efficiency, and ensures the blood is handled in a closed environment throughout the process, reducing environmental contamination. It can be performed in ordinary settings. The authors seek a feasible, easy-to-operate, environmentally low-requirement, and low-cost separation method to facilitate the clinical popularization of PRP.

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