Feasibility of the use of boron-doped diamond electrode coupled to electroanalytical techniques for the individual determination of pravastatin and its association with acetylsalicylic acid

Abstract

In this work, we explored the efficiency of the use of the pretreated boron-doped diamond electrode (BDDE) coupled to electroanalytical techniques for the individual determination of pravastatin (PRAV) and its association with acetylsalicylic acid (ASA). The voltammetric behavior of PRAV and ASA was investigated by cyclic voltammetry showing irreversible and diffusion-controlled oxidation processes for both analytes, regardless of the type of BDDE pretreatment. Under the optimized conditions (cathodically pretreated BDDE and phosphate buffer solution, pH 4.0), the analytical response for PRAV was linear over the range from 1.0 to 30 μmol L−1 for individual determination using amperometry, obtaining a limit of detection (LOD) of 0.102 μmol L−1. For simultaneous determination of PRAV and ASA, (using the anodically pretreated BDDE in 0.3 mol L−1 H2SO4 solution), the responses were linear over the range of 1.08–16.4 μmol L−1 for PRAV and 3.96–42.75 μmol L−1 for ASA using SWV, obtaining a LOD of 0.204 and 0.078 μmol L−1, respectively. The methods were successfully applied to pharmaceutical formulations, synthetic urine and tap water samples.