Abstract
Abstract The in vitro propagation of orchids is the only commercial large scale technique to obtain healthy and high quality plantlets with clonal origin. The use of new technologies in plant tissue culture systems could lead to efficiency increases and costs reduction of micropropagation systems. The main actual micropropagation system is based on semi-solid culture media solidified using agar, followed by sterilization using autoclaving, and cultivation under photomixotrophic conditions using sucrose as main source of energy to plant in vitro culture. We proposed in this study the use of new micropropagation system using chemical sterilized liquid medium using polyurethane foam as support and LED source of light in rooting stage of Miltassia ‘Shelobie Tolkien’. Thus, the objective of this research was to test different concentrations of sucrose, comparing the conventional semi-solid agar-based culture medium (control) and the use of liquid medium with polyurethane foam support. The following sucrose concentrations were used: 0, 7.5, 15, 22.5 and 30 g L-1. The experiment was conducted in a 2 × 5 factorial, in a completely randomized design with ten replications each over a total period of 105 days of cultivation. The chemical sterilization using ClO2 showed 100% of decontamination in all treatments. The use of liquid media with polyurethane foam showed better results than plants cultivated in agar medium, and can be used for replace agar-based for orchid in vitro rooting.
Highlights
The Orchidaceae family represents about 25,000 species, as well as thousands of hybrids around the world, being the largest family among monocotyledons (Zahara et al, 2017)
All the shoots used in the experiment originated from multiplication phase of in vitro micropropagation, obtained from shoot-tip culture, of clonal origin and obtained from a protocol developed in the Laboratory of Plant Physiology and Tissue Culture (LFVCT) of the Center of Agricultural Sciences of Federal University of São Carlos (UFSCar)
The experiment tested the physical state of the culture medium and the sucrose concentrations
Summary
The Orchidaceae family represents about 25,000 species, as well as thousands of hybrids around the world, being the largest family among monocotyledons (Zahara et al, 2017). The commercial production of ornamental orchids utilizes for propagation asymbiotic in vitro germination and cloning techniques of plants as the only techniques with commercial feasibility to obtain healthy and high quality plantlets in a large scale production This technique was applied in the production of species that are environmentally vulnerable or endangered (Datta et al 2017; Faveta et al, 2017). The Murashige and Skoog (1962) medium (MS) is the most used for the in vitro propagation of orchids, and depending of the species or hybrid used for micropropagation, the macronutrient concentrations of the medium are modified, reduced by half (1⁄2MS) to meet the plant requirements necessary for in vitro development of plantlets (Silva et al 2015). These modifications resulted in better development of micropropagated plantlets, but in most of the experiments these modifications are limited to nutrients, including treatments with types and concentrations of plant growth regulators, such as cytokinins and auxins (Park and Yeung, 2017)
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