Hatching of common carp ( Cyprinus carpio L.) embryos stored at 4 and −2 °C in different concentrations of methanol and sucrose

Abstract

The hatching performance of common carp ( Cyprinus carpio L.) embryos was examined after 12–72-h storage at 4 and −2 °C using different concentrations of sucrose (0.1, 0.25, 0.5 and 1.0 M or 3.42, 8.55, 17.10 and 34.2%), methanol (MeOH) (0.5, 1.0, 1.5, 2.0, 2.5, 3.0 and 3.5 M or 1.6, 3.2, 4.8, 6.4, 8.0, 9.6 and 11.2%), or varying concentrations of methanol in 0.5 M (17.10%) sucrose. For sucrose, 0.5 M (17.10%) showed the maximum survival (41±1% (12 h) to 11±1.5% (72 h)) at 4 °C. No survival was observed at −2 °C with any concentration of sucrose. At both temperatures employed, hatching was higher with mixed combination of methanol (1.5 M or 4.8%) and 0.5 M (17.10%) sucrose (4 °C: 41±1.5% (12 h), 38±1.2% (72 h); −2 °C: 33±1.7% (12 h), 28±1.2% (72 h)) compared to methanol alone (4 °C: 38±1.5% (12 h), 35±2.5% (72 h); −2 °C: 31±2.5% (12 h), 25±2% (72 h)). The combination of 1.5 M (4.8%) methanol and 0.5 M (17.10%) sucrose produced the best results among all the concentrations tested at both temperatures.