FDPS-Influenced Transcriptome Alternations in MC3T3-E1 Cells are Associated with the Pathogenesis of Osteoporosis

Abstract

Regulatory factors function by modulating a variety of cascade mechanisims in cells. FDPS is a potential RNA-Binding Protein involved in various biological functions such as transcription, post-transcriptional processing, translation and post-translational modification. FDPS promote post-translational modification of GTPase for the treatment of diseases associated with bone resorption. However, it is not clear whether FDPS plays an information-regulating role through the action of splicing factors. In this study, the cell biology, gene expression profile and alternative splicing pattern of FDPS-overexpressed MC3T3-E1 cells (FDPS-OE) were compared with the control. The results showed that FDPS-OE promoted cell proliferation and inhibited cell apoptosis, and identified the potential target genes and potential functions of FDPS. In addition, FDPS-OE extensively regulated the transcriptional levels of cellular immune response and glycometabolism-related genes, thus affecting the immune function of bone formation and glycometabolism of bone cells. Finally, FDPS extensively regulates the selective splicing of hundreds of genes, and has functions of regulating cell cycle, cell division and positive transcription. The splicing of these genes regulated by FDPS plays an important role in cell proliferation and apoptosis and gene transcription regulation. In conclusion, FDPS can mediate the formation and apoptosis of abnormal osteoblasts through splicing regulation, thus causing the occurrence and development of osteoporosis.