Abstract
Six wild (A. campestris, L. sulphureus, T. clypeatus, T. microcarpus, T. letestui and Termitomyces spps) and three cultivated (P. ostreatus, L. edodes, A. bisporus) edible mushrooms collected from Ethiopia were analyzed for their fatty acid profile. Fatty acids were extracted by one step lipid extraction and methylation procedure followed by gas chromatography with flame ionization detection. The dominant fatty acid in all mushroom species was linoleic acid (C18:2) ranging from 1044.5-2759.4 mg/100 g. The next three dominant fatty acid were oleic acid (C18:1n9c), palmitic acid (C16:0) and stearic acid (C18:0) ranging from 43.8-1558.8, 189.9-1081.5 and 13.5-374.1 mg/100 g respectively. Beside the four major fatty acids already described, more than 20 fatty acids were identified and quantified. The proportions of unsaturated fatty acids were of higher concentration than those of saturated fatty acids for all mushrooms. Moreover, the ratio of linoleic/oleic acid in all species are significantly different (P<0.05) and were greater than one.
Highlights
Fatty acid compositions have beneficial effects on blood lipid profiles
All of the methods devised for the preparation of fatty acid methyl esters using either acid-or basecatalyzed them are time consuming and impractical for processing a high number of samples because lipids have to be extracted prior to FAMES preparation
The mushroom were selected purposefully and evaluated for their fatty acid composition by a one step fat extraction and methylation followed by gas chromatography-flame ionization detection (GCFID) method of Garces and Mancha [4]
Summary
Fatty acid compositions have beneficial effects on blood lipid profiles. Analysis of fatty acid composition by GC usually requires derivatization of fatty acids to increase their volatility. Fatty acid methyl esters (FAME) may be prepared by different Tran’s methylation techniques and separated on GC columns and detected by flame ionization detection (FID) [3]. All of the methods devised for the preparation of fatty acid methyl esters using either acid-or basecatalyzed them are time consuming and impractical for processing a high number of samples because lipids have to be extracted prior to FAMES preparation. Garces and Mancha [4] have developed a convenient general method using complex reagent mixture for the digestion of the tissue, lipid Tran’s methylation and FAMES extraction in one step
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