Abstract
The purpose of this study was to identify fatty acid composition in fat extracted from rats using Gas Chromatography Mass Spectrometry (GC-MS) method. The fatty acid composition was determined as the methyl esters of fatty acids. Fat was extracted with chloroform-methanol solvent. Fatty acid methyl esters were prepared by saponification with base and followed by BF3-catalyzed methylation. Thirty compounds were identified, representing about 100 % of the total extracted lipids as measured by GC peak areas. The major constituents were 9-Octadecenoic acid; Hexadecanoic acid; 9,12-Octadecadienoic acid; Octadecanoic acid; 9-Hexadecenoic acid; Tetradecanoic acid; and Methyl arachidonate. The major constituents were fatty acids with chain lengths from 15 to 21 carbon atoms (mainly C17 and C19).
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: IOP Conference Series: Materials Science and Engineering
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
