Fast Immobilization of Human Carbonic Anhydrase II on Ni-Based Metal-Organic Framework Nanorods with High Catalytic Performance

Mengzhao Jiao,Frank Vriesekoop,Qipeng Yuan,Yanhui Liu,Jie He,Hao Liang,Shanshan Sun

doi:10.3390/catal10040401

Mengzhao Jiao, Frank Vriesekoop + Show 5 more

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https://doi.org/10.3390/catal10040401

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Abstract

Carbonic anhydrase (CA) has received considerable attention for its ability to capture carbon dioxide efficiently. This study reports a simple strategy for immobilizing recombinant carbonic anhydrase II from human (hCA II) on Ni-based MOFs (Ni-BTC) nanorods, which was readily achieved in a one-pot immobilization of His-tagged hCA II (His-hCA II). Consequently, His-hCA II from cell lysate could obtain an activity recovery of 99% under optimal conditions. After storing for 10 days, the immobilized His-hCA II maintained 40% activity while the free enzyme lost 91% activity. Furthermore, during the hydrolysis of p-nitrophenyl acetic acid, immobilized His-hCA II exhibited excellent reusability and still retained more than 65% of the original activity after eight cycles. In addition, we also found that Ni-BTC had no fixation effect on proteins without histidine-tag. These results show that the Ni-BTC MOFs have a great potential with high efficiency for and specific binding of immobilized enzymes.

Highlights

Carbonic anhydrase (CA, E.C. 4.2.1.1), a zinc metalloenzyme, can efficiently catalyze the reversible hydration of CO2 to bicarbonate ion [1,2]
Transmission electron microscopy (TEM) and Scanning electron microscopy (SEM) were used to characterize the morphology of Ni-BTC
We investigated whether the different monomers in His-hCA II @Ni-BTC could accelerate the relative catalytic activity at equal protein concentrations of His-hCA II @Ni-BTC and His-hCA

Summary

Introduction

Carbonic anhydrase (CA, E.C. 4.2.1.1), a zinc metalloenzyme, can efficiently catalyze the reversible hydration of CO2 to bicarbonate ion [1,2]. With the intensifying greenhouse effect, the use of carbonic anhydrase is considered as a green and efficient strategy for bio-mineralization of CO2 [4,5,6,7]. The poor stability and reusability of carbonic anhydrase limits its application in practice [6,8]. Previous immobilization studies mainly focused on commercial carbonic anhydrase on a wide range of solid supports such as: polyurethane foam [14], SBA-15 [15], mesoporous silica [16], MIL-160 [9] and ZIF-8 [17]. High cost and protracted immobilization processes greatly limit the application of immobilized CA [4,10,12]

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