Fast atrazine degradation by the mixed cultures enriched from activated sludge and analysis of their microbial community succession.

Abstract

In this study, fast atrazine degradation by the mixed bacterial cultures from sewage sludge was investigated. The acquired activated cultures showed great capability in atrazine degradation. The biodegradation process was well fitted into a pseudo-first reaction kinetic model. Atrazine could inhibit the propagation of ammonium oxidation bacteria and nitrite oxidation bacteria, decreasing the ammonium removal rate and the accumulation of nitrite. Only 162-172 reads of Nitrosomonadaceae and no Nitrospirales were detected after atrazine was exposed to the mixed cultures. The bacterial community structures in the cultures under different inoculation conditions (with or without atrazine) were investigated to explore the mechanism of atrazine degradation. Our results show that the genera Thiobacillus and Ferruginibacter were the most possible candidates responsible for the degradation of atrazine.