Farklı MS Dozlarının Buğdayda (Triticum sp.) Doku Kültürü Parametrelerine Etkileri

Abstract

Increasing crop production yield and crop losses caused by biotic-abiotic stress factors increased the attention to biotechnology-supported breeding studies. However, in these studies the cost of tissue culture and genetic engineering researches are considerably high and chemical substances constitute the major part of this cost. MS medium that provide in vitro nutrition to the explants in such studies is one of these frequently used chemicals. The purpose of this study is to determine the most economical MS medium dose that is necessary for obtaining plants through callus culture. Saricanak-98 durum wheat and Pehlivan common wheat cultivated varieties were used as plant material and mature embryos were moved slightly in the seeds for callus formation and were cultured in a solution containing 8 mg/l 2,4-D. Callus that developed in subsequent sub-cultures were cultured in Petri dishes containing MS medium in full (4,405 g/l), half (2,202 g/l) and quarter (1,101 g/l) doses. The weights of the calli were determined by measuring after 11 days and the number of plants obtained at the end of 7 weeks was recorded. After the statistical analysis it was found that different MS doses had no effect on the regeneration capacity and the number of plants obtained. During the formation of callus in solution and by using quarter MS dose in the following phase, it is possible to regenerate complete plants. By this way, MS medium used in sub-cultures which are common in gene transfer studies can be reduced by 75%.