Abstract
In this study, physiological responses of brook trout (Salmo trutta fario) were researched after the different stunning methods with biochemical enzymes. Icy water, electroshock, CO2, hypoxia, and head hitting were tried as stunning techniques to 75 fish and the alterations in the blood biochemistry parameters were analyzed. Alkaline phosphates (ALP), and amylase (AMS) were found as not statistically important, but alanine transaminases (ALT), aspartate transaminases (AST), creatin chitinase (CK), CK-MB (one of the CK isoenzymes), gamma glutamil transferase (GGT) and lactate dehydrogenase (LDH) levels were important (p<0.05). According to these results, the different stunning techniques affected the stress levels and physiological situations of brook trout. The study findings showed that killing techniques resulting in a shorter period were more suitable for animal welfare.
Highlights
Biological and environmental factors may cause stress in aquatic livings
Because of fish blood results of the stress response in the right behalf of developing secondary effects of stress factors and ecosystem health, a fast and complete results (Kayhan et al 2009; Atamanalp et al 2012) Biochemical reactions are catalyzed by enzymes (Kıran et al 2006)
The effects of different stunning methods on the biochemical parameters of brown trout (S. t. fario) blood are shown in Tables 1 and 2
Summary
Biological and environmental factors may cause stress in aquatic livings. These stress factors may be vital activities as well as various farm practices. Farm treatments, environmental factors, growth, malnutrition, hunger, stock and transport density, and deformities can be effective in the decreasein fish welfare (Conte 2004; El-khaldi 2010). Because of fish blood results of the stress response in the right behalf of developing secondary effects of stress factors and ecosystem health, a fast and complete results (Kayhan et al 2009; Atamanalp et al 2012) Biochemical reactions are catalyzed by enzymes (Kıran et al 2006). Stress factors provide expression with various metabolic findings such as hormonal, enzymatic and blood parameter alterations.
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