Farklı bakteriyel kontaminasyonun sıçanlarda deneysel adeziv barsak obstrüksiyonuna etkisi

Abstract

Aim: Postoperative peritoneal adhesions (PPA) cause pain, intestinal obstruction and infertility after abdominal surgery and to date there is no shown pathogenesis or definitive treatment. One of the most important points in intraabdominal interventions is bowel flora and its effect on infection. It was thought that different microorganisms found in flora or frequently added as hospital flora might cause inflammatory processes and cause PPA formation. There are a lot of studies showing intraabdominal infections cause PPA but there is no study comparing the effect of different bacterial strains on PPA formation. That is why we designed this study using the most common microorganisms isolated in intraabdominal infections and hospital flora. In this study, we aimed to investigate the effect of different bacterial strains (Klebsiella spp, E. coli spp, anaerobe) on the formation and degree of PPA in adhesion formation in rats. Methods: In this study, rats were divided into 5 groups, consisting of twelve rats. Groups were classified as; E. coli, Klebsiella, Bacteriodes fragilis, Sham and Control groups. The rats were sacrificed on the fourteenth day and relaparotomy was performed. The results were evaluated macroscopically and microscopically according to the previously determined classifications. Results: When bacterial infected groups were compared with sham and control groups, both microscopic and macroscopically significant increase in PPA was observed. Conclusion: In the experimentally generated adhesion model, microorganisms have been found to play an active role in PPA formation. But there was no significant difference between bacterial strains on PPA formation.