Abstract
We have developed a technique which allows us to determine the location of a fluorescent signal to within 10nm accuracy within a topological scan created by AFM. Imaging topological markers that are both fluorescent and resolvable in an AFM scan, we can triangulate the fluorescence of these markers in conjunction with the fluorescence and topology of a sample to within 10nm accuracy. Here we show data from this method in air as well as wet buffers which are ideal for biological sample measurements. This technique will facilitate localization of fluorescent molecules within asymmetric diffraction limited biological samples.
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