Abstract
Nucleic acid-based tests are rapidly expanding in the field of diagnostic microbiology, due to their unique high sensitivity and specificity as well as rapid assay turnaround time. However, the potential of false positives and false negatives can hinder the wide application of these novel techniques. This mini-review article summarizes common causes and potential solutions for false-positives and false-negatives encountered in the field of diagnostic molecular microbiology.
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