FACULTATIVELY-ANAEROBIC ENDOSPOREFORMING BACTERIA OF DEEP WATER BOTTOM SEDIMENTS OF BLACK SEA

Abstract

Aim of the work was to detect in deep-sea sediments of the hydrogen sulfide bathyal zone of the Black Sea the facultative-anaerobic spore-forming bacteria (FASB) capable of grow under aerobic conditions, and to determine their taxonomic diversity. Methods. For the work were used samples of bottom sediments from different horizons (0-50 cm) from the depth of 888-2080 m of the Black Sea, collected during the expedition of the M84/2 of the University of Bremen to the Meteor ship. Identification of isolated strains was carried out by determining the spectra of fatty acids on a BioRad gas chromatograph using an automatic microorganism identification system MIDI Sherlock based on a gas chromatograph with Agilent 7890 flame ionization detector (Agilent Technologies, USA). Results. In the course of research in deep-sea sediments of the hydrogen sulfide bathyal zone of the Black Sea on horizons up to 50 cm were found facultative anaerobic bacteria, capable of grow under aerobic conditions. Were obtained 202 isolates of endosporeforing gram-positive bacteria. According to the spectra of fatty acids using the automatic identification system for microorganisms, MIDI Sherlock found that the resulting isolates belong to 18 Species of 4 genera (FAEB): Bacillus, Paenibacillus, Lysinibacillus, Brevibacillus. About 20 unidentified isolates require additional research and can refer to new bacteria that are not known for science yet. Conclusions. For the first time in the bottom sediments on the horizons up to 50 cm of anaerobic hydrogen sulfide and methane bathyal zone of the Black Sea at depths 888-2080 m, were identified the facultative anaerobic spore-forming bacteria, their taxonomic diversity was determined, and were made assumptions about their alochtonous origin.