Abstract
SUMMARYA technique has been evolved whereby the rate of attachment or adherence of bacteria to mouse peritoneal macrophages can be studied.The aim of the technique was to obtain samples, taken at intervals after mixing a suspension of bacteria and macrophages, which contained bacteria not yet associated with cells. This was achieved by passing the test suspensions through filters holding back macrophages but allowing tin‐ free passage of bacteria not attached to these cells.Using this method no association of S. typhimurium C5 with macrophages occurs unless the organisms have been opsonised. It has also been shown that there are one or more heat‐labile antigens present on the surface of this organism. These must be covered before the organism will adhere to the surface of mouse macrophages.
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