誘導マクロファージの Ia 抗原に関する研究 : 第 2 報活性化 T 細胞接種による Ia 抗原量の変化について

Abstract

It is known that quantity of Ia antigen on the surface of macrophages increases in the presence of stimulated T cells in cell culture. Murine spleen T cells and thymus T cells were stimulated with Concanavalin A. The T cells were injected in murine peritoneum, and then the quantity of Ia antigen on the surface of peritoneal macrophages was measured using the method of enzyme-linked immunosorbent assay (ELISA). The results were as follows ; 1. In the presence of stimulated thymus T cells, the quantity of Ia antigen did not increase on the surface of macrophages. 2. In the presence of stimulated spleen T cells, the quantity of Ia antigen increased on the surface of macrophages. 3. On the surface of resident macrophages, the quantity of Ia antigen did not increase after injection of stimulated T cells. 4. On the surface of Pepton-induced macrophages, the quantity of Ia antigen increased rapidly, but soon decreased, after injection of stimulated spleen T cells. 5. On the surface of LPS-induced macrophages, the quantity of Ia antigen decreased slightly, and then increased gradually, after injection of stimulated spleen T cells. These results showed that stimulated spleen T cells had the capacity for increasing the quantity of Ia antigen on the surface of macrophage in mice. The expression of Ia antigen on the surface of macrophages differed with the conditions of macrophages.