Abstract
SummaryThe nutritional requirements of marine bacteria are so varied that no one nutrient medium can suffice for the growth of all. The influence of composition of the nutrient medium and plating procedure on the plate method for determining the number of bacteria in sea water was investigated. Seven media were tested. Maximum and reproducible plate counts were obtained with a medium containing 0.5% peptone, 0.01% yeast extract, 0.01% FePO4, and 1.5% agar in 80% sea water. The number of colonies that developed on pour plates was 30–40% greater than the number that developed on surface inoculated plates.
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