Abstract
The interaction of a potent carcinogen, aflatoxin B 1 (AFB 1), with a probiotic strain of lactic acid bacteria, Lactobacillus rhamnosus strain GG (GG), has been investigated. The binding of AFB 1 to GG in the late exponential–early stationary phase was studied for viable, heat-killed and acid-killed bacteria. In general, viable, heat-killed and acid-killed GG responded in a similar manner. The effects of pronase E, lipase and m-periodate on AFB 1 binding and release were consistent with AFB 1 binding predominantly to carbohydrate components of the bacteria. The effect of urea suggested hydrophobic interactions play a major role in binding. Increasing concentration (0.01–1 M) of NaCl or CaCl 2 had minor effects on AFB 1 binding suggesting some involvement of electrostatic interactions. An increase in pH from 2.5 to 8.5 had no effect on AFB 1 binding but decreased binding of AFB 2a, possibly due to hydrogen bonding interactions.
Published Version
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