Abstract
An evaluation was made of a modification of the Sumerwell and Sealock procedure for the determination of bound ascorbic acid. It was demonstrated that the amount of bound ascorbic acid determined to be present in blood serum or plasma depended upon the time and temperature of hydrolysis, the sample size, and the presence or absence of several constituents of blood or tissues. Attempts to demonstrate the presence of bound ascorbic acid in blood serum by means of autolysis or enzymic hydrolysis were unsuccessful. It is doubtful that existing analytical procedures are sufficiently specific to demonstrate conclusively the existence of bound ascorbic acid in animal tissues or fluids.
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