Factors Affecting Shellfish Quality in Terms of Faecal Contamination at Blakeney Point, East Anglia, UK

Andrew S Ball,Esmaeil Shahsavari,David J Smith,Arturo Aburto-Medina,Leadin S Khudur

doi:10.3390/w13223192

Abstract

Relatively high E. coli and Faecal Streptococci (FS) numbers have been reported in the waters surrounding Blakeney Point, East Anglia, UK, an area containing significant shellfishery industries including mussels and cockles, despite the implementation of development works aimed at reducing residual contamination problems. The aim of this study was to evaluate the potential of using bacterial analyses and sterol profiling to assess the current levels and source of faecal pollution at Blakeney Point. No evidence of significant human faecal contamination of water in and around Blakeney Point could be found using either traditional microbiological analyses or sterol profile analysis. The presence of significant quantities of sitosterol, however, suggests that faecal contamination of water from birds does occur but at the concentration detected would not affect water quality. Analysis of cockles and mussels taken from the area show that negligible levels of coprostanol were present, confirming that faecal pollution was not causing any contamination issues. Apart from cholesterol, brassicasterol, an algal biomarker, as expected was dominant in shellfish flesh. The results confirm that current water treatment processes are successful in ensuring water quality at Blakeney Point and that a combination of microbial testing with sterol profile analysis confirmed that low microbial concentrations of faecal contaminants present in and around Blakeney Point most probably originate from migratory and/or resident bird species.

Highlights

Bacterial indicators of faecal pollution, such as the faecal coliforms (FC)/faecal streptococci (FS) ratio, have been extensively used to quantify faecal material entering aquatic systems [1]
Compared to many inland waters affected by faecal contamination, the E. coli concentrations are low in most samples examined (Figure 2a)
E. coli and Faecal Streptococci (FS) numbers have been reported in the waters surrounding Blakeney Point, East Anglia, UK, an area containing significant shellfishery industries, including mussels and cockles, despite the implementation of remedial works aimed at reducing residual contamination problems

Summary

Introduction

Bacterial indicators of faecal pollution, such as the faecal coliforms (FC)/faecal streptococci (FS) ratio, have been extensively used to quantify faecal material entering aquatic systems [1]. The main origins of faecal contamination found in rivers, streams, and estuaries tend to be from the release of untreated sewage or treated wastewater effluent, stormwater drains, industrial effluents, and run-off from adjacent animal farms [2,3,4,5]. The application of bacterial indicators to identify sources of faecal pollution is difficult due to the effect of different environmental conditions such as temperature and salinity, which significantly affect bacterial survival rates [7,8,9,10]. Neither FC or FS or their ratio can be used to identify sources of faecal pollution as there is no one species restricted to a single animal [11,12,13]

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Conclusion