Factors Affecting Regeneration of Tomato Cotyledons

Abstract

In this research, we employed the tomato cultivar named Zhongshu Si Hao as research material to study the influences on the regeneration of adventitious buds under the different conditions with hormone combinations, various explants, different parts of cotyledons and cotyledon inoculatingmethods; we also screened the best method for seed surface disinfection and the most appropriate medium for the rooting of adventitious buds. The results showed that the best procedures for surface disinfection of tomato seeds would be as follows: firstly, immersing the seeds in 70% ethanol for 30 second, then rinsing them in 20% NaClO with tween-20 for 20 min. The most ideal explant for inducing buds is the middle part of the cotyledon, followed by the stem and the leaf. the best medium formula for cotyledon regeneration is MS+ZT 2 mg/L+IAA 0.1 mg/L, and placing the cotyledon upside down is better to induce the adventitious buds than that placing upside. The best medium for rooting of adventitious buds is 1/2MS+IAA 0.3 mg/L. This research would lay a foundation establish transformation system of tomato in our future research.