Abstract
The effect of environmental factors on nitrogenase activity and on transcription of nif genes in Azospirillum brasilense was investigated. Addition of ammonium, glutamine, oxygen, nitrate or nitrite together with either iron limitation or shift of temperature caused different levels of inhibition of nitrogenase activity. The nif mRNA was completely absent after addition of either ammonium or glutamine and after iron deprivation, while oxygen, nitrate or nitrite partially decreased the presence of nif mRNA. It is concluded that in A. brasilense the control of the nitrogenase switch-off is independent of the transcription of nif genes.
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