Abstract
A partly purified extract of the skin of the Australian frog Pseudophryne coriacea (PsC) evoked the release of [ 3H]acetylcholine ([ 3H]ACh) and of [ 3H]5-hydroxytryptamine ([ 3H]5-HT) from superfused rat cerebral cortex synaptosomes prelabeled with [ 3H]choline or [ 3H]5-HT, respectively. The PsC-evoked release of both transmitters was sensitive to tetrodotoxin and was strictly Ca 2+-dependent. The release of [ 3H]5-HT caused by PsC was unaffected by the 5-HT uptake inhibitor citalopram. Activation of muscarinic autoreceptors by ACh or of serotonin autoreceptors by 5-HT depressed the PsC-evoked release of [ 3H]ACh or of [ 3H]5-HT, respectively. It is concluded that PsC elicits a Ca 2+-dependent exocytotic-like transmitter release, possibly by opening Na + channels in the presynaptic membrane.
Published Version
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