Facile green synthesis and characterization of Crataegus microphylla extract-capped silver nanoparticles (CME@Ag-NPs) and its potential antibacterial and anticancer activities against AGS and MCF-7 human cancer cells

Abstract

A facile, fast and inexpensive method to preparation of silver nanoparticles (CME@Ag-NPs) with very favorable efficiency in biological assay has been expanded utilizing Crataegus microphylla fruit extract as reducing agent. Some parameters such as pH, time, AgNO3 concentration and temperature on the biological assay efficiency and morphological characteristics of CME@Ag-NPs were investigated to reach optimum condition. FT-IR, XRD, TEM, DLS, FESEM, EDS and UV/Vis analysis were used to investigate the properties of the as-synthesized CME@Ag-NPs. Regular, homogeneous and spherical-like morphology of CME@Ag-NPs with a mean particle size of 30–50 nm was obtained from FESEM and TEM images. The antibacterial activity of CME@Ag-NPs was explored on Acinetobacter baumannii, Staphylococcus aureus, Enterococcus, Pseudomonas, Escherichia coli, Klebsiella and Proteus mirabilis bacteria by MIC and MBC test. Investigation of the antibacterial activity showed that as-obtained nanoparticles have significant activity against Proteus mirabilis (MIC value of 3.5 μg/ml and MBC value of 7 μg/ml) than other bacterial strains. Further, antibacterial activity of CME@Ag-NPs was determined against multidrug-resistant. Interestingly, as-synthesized silver nanoparticles displayed considerable antibacterial activity against all the test strains. In vitro cytotoxicity of nanoparticles on MCF-7 and AGS cell lines was assayed in different concentration of CME@Ag-NPs and doxorubicin as positive control. As the results show, CME@Ag-NPs is excellent effective in inhibiting the growth of MCF-7 and AGS cell lines.