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Abstract
We report on a simple way to produce three-dimensional (3D) microstructures embedded in microchannel which is used as a micromixer in a microfluidic device. The micromilling machine has great capability of easily transferring the microstructured matter design onto the device substrate. According to the determined microstructure design, the different channel depths, widths, and complicated designs of microstructure are easily realized by the micromilling machine and thermoplastic substrate. These results provide a cost-effective way to produce master molds or their replica for the generation of complicated 3D micromixer embedded microfluidic devices. Furthermore, the complicated microstructure is suitable to use as micromixer which is important in microfluidicbased applications.
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