Facial preparation and in vitro bioactivity of rice-like Mg doped CaCO3 particles for bone tissue regeneration

Abstract

Calcium carbonate (CaCO3) particles with controlled polymorphs, morphologies and specific sizes presented great application potential in biomedical field. In this work, CaCO3 particles with controlled morphologies and surface micro-structure were successfully synthesized by facile and eco-friendly processes. Results revealed that the morphology of CaCO3 could be controlled from spherical to rice-like and dumbbell shaped particles with different surface micro-structure by introducing Mg2+ and adjusting the molar ratio of Mg2+/Ca2+. Herein, the in vitro bio-activity of prepared rice-like CaCO3 particles was investigated by immersion in SBF solution for 1, 3 and 5 days. Moreover, in vitro cyto-compatibility of prepared rice-like CaCO3 particles has also been evaluated by CCK-8 and live/dead staining assay. Results demonstrated that the rice-like CaCO3 particles possess excellent bio-activity and in vitro cyto-compatibility, which suggest the great promise of prepared rice-like CaCO3 particles in bone tissue engineering.