Abstract
Due to their interesting ferroelectric, conductive and dielectric properties, in recent years, perovskite-structured materials have begun to attract increasing interest in the biosensing field. In this study, a strontium titanate perovskite layer (SrTiO3) has been synthesized on a platinum electrode and exploited for the development of an impedimetric label-free immunosensor for Escherichia coli O157:H7 detection. The electrochemical characterization of the perovskite-modified electrode during the construction of the immunosensor, as well as after the interaction with different E. coli O157:H7 concentrations, showed a reproducible decrease of the total capacitance of the system that was used for the analytical characterization of the immunosensor. Under optimized conditions, the capacitive immunosensor showed a linear relationship from to 1 to 7 log cfu/mL with a low detection limit of 1 log cfu/mL. Moreover, the atomic force microscopy (AFM) technique underlined the increase in roughness of the SrTiO3-modified electrode surface after antibody immobilization, as well as the effective presence of cells with the typical size of E. coli.
Highlights
The impact of biosensing technology is increasing in all major sectors, such as pharmaceuticals, healthcare, environment, and food
The design of label-free affinity-based probing concepts is the objective of much current research, at both academic and industrial levels, towards establishing alternative methods to the already-existing enzyme-linked immunosorbent assay (ELISA)-based immunoassays
Among the different types of immunosensors that enable the direct monitoring of such interactions, impedimetric immunosensors have recently received particular attention since they possess a number of attractive characteristics associated with the use of electrochemical transducers, namely, the low cost of electrode mass production, cost effective instrumentation, the ability to be miniaturized and to be integrated into multi-array or microprocessor-controlled diagnostic tools, remote control of implanted sensors, etc. [2,3]
Summary
The impact of biosensing technology is increasing in all major sectors, such as pharmaceuticals, healthcare, environment, and food. Due to their simple use and application in relatively complex samples, biosensors offer a potential alternative to the most advanced bioanalytical systems [1]. Among the different types of immunosensors that enable the direct monitoring of such interactions, impedimetric immunosensors have recently received particular attention since they possess a number of attractive characteristics associated with the use of electrochemical transducers, namely, the low cost of electrode mass production, cost effective instrumentation, the ability to be miniaturized and to be integrated into multi-array or microprocessor-controlled diagnostic tools, remote control of implanted sensors, etc. Among the different types of immunosensors that enable the direct monitoring of such interactions, impedimetric immunosensors have recently received particular attention since they possess a number of attractive characteristics associated with the use of electrochemical transducers, namely, the low cost of electrode mass production, cost effective instrumentation, the ability to be miniaturized and to be integrated into multi-array or microprocessor-controlled diagnostic tools, remote control of implanted sensors, etc. [2,3].
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