Abstract
A highly sensitive amperometric glucose sensor was developed by immobilization of glucose oxidase (GOx) onto multi-layer reduced graphene oxide (MRGO) sheets decorated with platinum and gold flower-like nanoparticles (PtAuNPs) modified Au substrate electrode. The fabricated MRGO/PtAuNPs modified hybrid electrode demonstrated high electrocatalytic activities toward oxidation of H2O2, to which it had a wide linear response that ranged from 0.5 to 8 mM (R2 = 0.997), and high sensitivity of 506.25 μA/mMcm2. Furthermore, glucose oxidase-chitosan composite and cationic polydiallyldimethylammonium chloride (PDDA) were assembled by a casting method on the surface of MRGO/PtAuNPs modified electrode. This as-fabricated hybrid biosensor electrode exhibited high electrocatalytic activity for the detection of glucose in PBS. It demonstrated good analytical properties in terms of a low detection limit of 1 μM (signal-to-noise ratio of 3), short response time (3 s), high sensitivity (17.85 μA/mMcm2), and a wide linear range (0.01–8 mM) for glucose sensing. These results reveal that the newly developed sensing electrode offers great promise for new type enzymatic biosensor applications.
Highlights
Effective and fast determination of glucose level is of substantial interest in many areas, including clinical diagnostics, bio and physiological research, as well as food companies [1, 2]
A number of layers of Multi-layered reduced graphene oxide (MRGO) sheet were found on the Au seed layer, and the crumpled structure of the MRGO sheets constructed during chemical reduction is evident
These two figures show that no structural change occurs to the monometallic nanoparticles on the MRGO modified surface
Summary
Effective and fast determination of glucose level is of substantial interest in many areas, including clinical diagnostics, bio and physiological research, as well as food companies [1, 2]. The CV of the MRGO/PtAuNPs-modified hybrid electrode in the 2 mM H2O2 solution is shown Fig 4a(iv). The amperometric responses of MRGO and MRGO/PtAuNPs modified hybrid electrodes, respectively, at +0.6 V upon consecutive drops of H2O2 in PBS solution, together with the calibration curves inserted in the inset of the figures as shown Fig 4b(i) and 4b(ii).
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