Fabrication of molecular imprinting electrochemical sensor for determination of 2-O-(β-d-glucopyranosyl) ascorbic acid

Abstract

2-O-(β-D-glucopyranosyl (AA2βG) is a natural component unique to Lycium barbarum (goji berry). The detection of its content can identify the quality of Goji berry extract and provide a new quality standard for Goji berry extract. Currently, there is only one report of electrochemical detection of AA2βG. Further improvement of electrochemical detection of AA2βG is necessary. Molecular imprinting polymer (MIP) is predetermined, identifiable and practical. The MIP-based electrochemical sensor has the advantages of high selectivity, fast response and easy operation. In this study, AuNPs and graphene composite was synthesized and modified the glassy carbon electrode, and 4-aminothiophenol (4-ATP) was self-assembled onto the AuNPs/GCE electrode surface by Au-S bonding, and then graphene was modified onto the electrode surface. 4-ATP and AA2βG were selected as the functional monomer and template molecule, respectively. Under optimal conditions, the sensor can achieve linear detection in the range of 100 nM to 10 μM and 10 μM to 100 μM, respectively. The detection limit can reach 27 nM.