Abstract
This paper presents a method of particle manipulation by dielectrophoresis (DEP) and immobilization using photo-crosslinkable resin inside microfluidic devices. High speed particle manipulation, including patterning and concentration control by DEP was demonstrated. Immovable and movable microstructures embedding particles were fabricated on-chip. Several microelectrodes were fabricated using Indium Tin Oxides (ITO) and Cr/Au. The two kinds of DEP responses of yeast cells (W303) and other particles were experimentally confirmed. Based on negative DEP phenomenon, cell traps generated by microelectrodes were demonstrated. Position control, transportation and patterning of cells were performed with cell traps. The on-chip fabrication of arbitrary shapes of microstructures based on Poly(ethylene glycol) diacrylate (PEGDA) was reported. With cell patterning by DEP and immobilization using on-chip fabrication, microstructures embedding line patterned cells were fabricated inside microfluidic channels. A novel m...
Highlights
The research about high‐throughput cell assembly is taken very seriously, since it can provide effective approaches for the construction of implantable in vitro artificial tissue for tissue engineering [1]
We presented a new method of fabricating movable microstructures embedding controllable particles inside microfluidic devices
It was combined with micromanipulation using DEP and immobilization using photo‐crosslinkable resin
Summary
The research about high‐throughput cell assembly is taken very seriously, since it can provide effective approaches for the construction of implantable in vitro artificial tissue for tissue engineering [1]. 1ic0r,o1st3ru2c:2tu0r1e3s 1 Embedding Controllable Particles Inside Dielectrophoretic Microfluidic Devices surface adhesion as in reference [6], it is possible to manipulate cells without causing damage. We present a method of manipulating particles and cells by DEP and immobilizing them using photo‐ crosslinkable resin inside microfluidic devices. The patterned particles and cells are immobilized inside microstructures and can be further assembled into complex three dimensional (3D) structures. There are cell embedded PEGDA structures patterned by DEP as seen in [22]. This approach includes some open chamber manipulation which is subject to contamination. On the other hand, movable microstructures embedding controllable cells can be directly fabricated inside microfluidic channels. These movable microstructures can be further assembled into more complex 3D structures and could become functional components of artificial tissue
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