Abstract
The current goal of cell sheet engineering technology is to provide an improved approach for more predictive and effective generation of viable tissue-like constructs. Success requires validation and characterization of cell sheet growth and detachment. In this study, we perform qualitative and quantitative assessments of cell sheets lifted from thin spin-coated poly(N-isopropylacrylamide)films.
Highlights
Cell sheet tissue engineering technologies have been rapidly developed and applied in different areas of biomedical research, including regenerative medicine [13], cell-based drug screening assays [4,5], and tissue and disease modelling [6,7,8,9]
This study demonstrates the potential of spin-coated poly(N-isopropylacrylamide) films in producing viable cell sheets that might be considered as a building block to create large biological tissues
One-week Human Corneal Epithelial Cells (HCEC) did not detach as an intact cell sheet, and only those HCEC cultured on pNIPAm surface for 2 weeks were able to detach as an intact sheet within around 40 min
Summary
Cell sheet tissue engineering technologies have been rapidly developed and applied in different areas of biomedical research, including regenerative medicine [13], cell-based drug screening assays [4,5], and tissue and disease modelling [6,7,8,9]. Most experimental studies have used cell sheets for scaffold-free tissue engineering Page 1 SCHOLARLY PAGES. Several studies have shown that cell sheet transplantation has been effective in the treatment of severe diseases such as cardiomyopathy and congestive heart failure [13], neovascular age-related macular degeneration [14], many forms of liver disease [15], diabetes mellitus [16], massive burns [17] and cartilage degeneration and defects [18]. Because cell sheets can be transplanted without sutures, this procedure saves time, reduces risk from biological materials and can avoid suture-related problems, such as inflammation and scars. This approach offers several distinctive therapeutic advantages, especially for cornea, esophageal epithelium or oral mucosa regeneration [19,20,21]
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