Fabrication of chitosan nanoparticles for the determination of phenylisatin and its analytical application in biological sample

Abstract

The synthesis of 1Phenylisatin (Ph) incorporated chitosan nanoparticles (Ph CSNPs) was done utilizing the conventional sol–gel method. For the characterization of CSNPs and Ph CSNPs, different techniques like FTIR, SEM, XRD and TEM were utilized. The Ph CSNPs and the pure Ph were interacted with bovine serum albumin (BSA). The results of fluorescence quenching confirm that both the systems are static in nature. The decreased Ksv value for the nanoparticles confirms the increased remedial efficiency of the Ph CSNPs and therefore the synthesized CSNPs can be used as a drug delivery system. The developed fluorescence-based method also reports the quantification and detection limit values for the Ph molecule. The linear dynamic range after the optimization of experimental conditions was found to be 2.91–6.54 µg mL−1. The values of limit of detection (LOD) and quantification (LOQ) for pure Ph system are 0.462 µg mL−1 and 1.54 µg mL−1 respectively, while for the Ph CSNPs system the values are 0.560 µg mL−1 (LOD) and 1.86 µg mL−1 (LOQ). These values were reported with high correlation coefficient values.