Abstract
Antheraea pernyi silk fibroin (ASF)-based nanofibers have wide potential for biomaterial applications due to superior biocompatibility. It is not clear whether the ASF-based nanofibers scaffold can be used as an in vitro cancer cell culture platform. In the current study, we fabricated novel ASF-based thermoresponsive hydrogel nanofibers by aqueous electrospinning for colon cancer (LoVo) cells culture. ASF was reacted with allyl glycidyl ether (AGE) for the preparation of allyl silk fibroin (ASF-AGE), which provided the possibility of copolymerization with allyl monomer. The investigation of ASF-AGE structure by 1H NMR revealed that reactive allyl groups were successfully linked with ASF. ASF-based thermoresponsive hydrogel nanofibers (p (ASF-AGE-NIPAAm)) were successfully manufactured by aqueous electrospinning with the polymerization of ASF and N-isopropylacrylamide (NIPAAm). The p (ASF-AGE-NIPAAm) spinning solution showed good spinnability with the increase of polymerization time, and uniform nanofibers were formed at the polymerization time of 360 min. The obtained hydrogel nanofibers exhibited good thermoresponsive that the LCST was similar with PNIPAAm at about 32 °C, and good degradability in protease XIV PBS solution. In addition, the cytocompatibility of colon cancer (LoVo) cells cultured in hydrogel nanofibers was assessed. It was demonstrated that LoVo cells grown on hydrogel nanofibers showed improved cell adhesion, proliferation, and viability than those on hydrogel. The results suggest that the p (ASF-AGE-NIPAAm) hydrogel nanofibers have potential application in LoVo cells culture in vitro. This study demonstrates the feasibility of fabricating ASF-based nanofibers to culture LoVo cancer cells that can potentially be used as an in vitro cancer cell culture platform.
Highlights
Cell culturing is a fundamental and essential process of biological sciences, for a wide range of basic and clinical in vitro research, which has led to outstanding developments in the fields of life science, biomedical, and biomaterial science [1]
Novel Antheraea pernyi silk fibroin (ASF)-based thermoresponsive hydrogel nanofibers were successfully fabricated by aqueous electrospinning for colon cancer (LoVo) cells culture
The p (ASF-AGENIPAAm) spinning solution showed good spinnability with increased polymerization, and uniform nanofibers were formed with a diameter of 452 ± 120 nm at the polymerization time of 360 min
Summary
Cell culturing is a fundamental and essential process of biological sciences, for a wide range of basic and clinical in vitro research, which has led to outstanding developments in the fields of life science, biomedical, and biomaterial science [1]. Investigation on formation, proliferation, invasion and metastasis are important research topics of malignant tumor In this condition, the fabrication of in vitro cancer cell culture models (analogous to scaffolds in tissue engineering) that mimic the microenvironment where the cancerous cells reside and the tumor develops are in high demand [3]. Hydrogels have been investigated as a reliable material for cell culture and tissue engineering due to their high water content that can mimic the extracellular matrix (ECM) and regulate cell function [4,5,6,7]. Electrospinning nanofibers are believed to be an efficient fabrication of in vitro cancer cell culture models for enhancing cell proliferation and adhesion
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