Fabrication of Ag nanoparticles mediated by extract of plant and determination of the ‎anti-human lung cancer effects

Abstract

IntroductionThe present work demonstrates the synthesis of Ag nanoparticles (Ag NPs) by aqueous extract of Thymus ‎capitatus as green reductant and capping agent without any toxic reagent. ‎Material and methodsPhysicochemical characteristics of the said nanocomposite were elucidated by field emission scanning electron ‎microscopy (FE-SEM), fourier-transform infrared spectroscopy (FTIR), and UV-Vis Spectroscopy. ‎ResultsThe biogenic Ag NPs are uniformly globular. The Ag NPs has been explored biologically in the anticancer and ‎antioxidant assays. In the cellular and molecular part of the recent study, the treated cells with Ag NPs were ‎assessed by MTT assay for 48h about the cytotoxicity and anti-human lung adenocarcinoma properties on ‎normal (HUVEC) and lung adenocarcinoma cell lines i.e. lung well-differentiated bronchogenic adenocarcinoma ‎‎(HLC-1), lung moderately differentiated adenocarcinoma (LC-2/ad), and lung poorly differentiated ‎adenocarcinoma (PC-14). The viability of malignant lung cell line reduced dose-dependently in the presence of ‎Ag NPs. The IC50 of Ag NPs were 209, 185, and 106 µg/mL against HLC-1, LC-2/ad, and PC-14 cell lines, ‎respectively. In the antioxidant test, the IC50 of Ag NPs and BHT against DPPH free radicals were 86 and 76 ‎‎µg/mL, respectively. ‎ConclusionsAfter clinical study, Ag NPs containing Thymus capitatus leaf aqueous extract may be used to formulate a new ‎chemotherapeutic drug or supplement to treat the several types of human lung adenocarcinoma. ‎