Fabrication and characterization of chitosan-pectin emulsion-filled hydrogel prepared by cold-set gelation to improve bioaccessibility of lipophilic bioactive compounds

Abstract

Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, incorporating a sodium caseinate-stabilized emulsion, was prepared using cold-set gelation under acidic conditions without crosslinking agents. Increasing the pectin concentration (0.75–1.50%, w/v) improved the mechanical strength and compactness of the EFH. The pH-responsive EFH retained the emulsion at pH 2.0 and released it at pH 7.4. In vitro digestion demonstrated that the EFH remained intact during oral and gastric stages, while the emulsion alone became destabilized. During intestinal digestion, the release of free fatty acids from the EFH decreased from 58.67% to 43.76% as the pectin concentration increased from 0.75% to 1.50%. EFH with 0.75% and 1.00% pectin significantly improved curcumin bioaccessibility compared to the emulsion alone. These findings demonstrate the potential of chitosan-pectin EFH as a novel carrier system for enhancing the bioaccessibility of lipophilic bioactive compounds.