F2-Isoprostane excretion rate and diurnal variation in human urine

Abstract

8-Iso-PGF2αis formed in vivo by non-enzymatic free radical catalysed oxidation of arachidonic acid. Urinary measurement of this compound has previously been shown to reflect the oxidative stress of the body in human and animal studies. To investigate the normal excretion rate and a possible diurnal variation of 8-iso-PGF2αexcretion in humans urinary samples were collected from ten healthy volunteers of both sexes at different times during a day and as a 24-h urine sample. The samples were analyzed by a newly developed radioimmunoassay with a specific antibody against free 8-iso-PGF2α. There was no diurnal variation in the urinary levels of 8-iso-PGF2αduring the day in this study. Neither was there any statistically significant difference between the 8-iso-PGF2αlevels at any time of the day or in the morning urine samples compared to the 24-h urine samples. In conclusion, all urine samples collected at any time of the day, preferably a morning urine sample (representative urine from 6-8 hours), can thus be used to obtain a reliable and adequate value of the amount of the 8-iso-PGF2αexcretion in urine in healthy individuals.