Abstract
Extraction, purification and preliminary characterization of polysaccharides from Hyriopsis cumingii (HCPS) were carried out. Firstly, the extracting parameters for HCPS extraction were optimized by using three-factor-three-level Box–Behnken design and response surface methodology based on the single-factor experiments. As results, the optimum conditions were extracting temperature 80 °C, extracting time 4.5 h and the ratio of extraction solvent (water) to raw material (v/w) 8. Under these conditions, the experimental yield of crude HCPS was 3.66%. Secondly, the crude HCPS was purified by DEAE-Cellulose 52 chromatography and Sephadex G-100 chromatography to afford 3 fractions, HCPS-1, HCPS-2 and HCPS-3. The recovery rates based on crude HCPS used were 26.91%, 30.18% and 3.93% for HCPS-1, HCPS-2 and HCPS-3 respectively. Based on the characterization results of HCPS by Fourier transform-infrared spectroscopy, high performance liquid chromatography and gas chromatography, we found that HCPS-3 was quite different from HCPS-1 or HCPS-2.
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