Abstract
Steroid sulphates are extracted from plasma in good yield by benzene/benzyltributylammonium chloride. Steroid glucuronides are not extracted. This method offers some advantages as compared with the direct solvolysis which is only to be recommended for the determination of steroid sulphates such as dehydroepiandrosterone sulphate which are present in plasma in higher concentrations. The coagulation of plasma proteins with ethanol has the disadvantage that steroid sulphates are adsorbed to the protein precipitated. Furthermore, there is no separation of steroid glucuronides. 17-Hydroxyprogesterone sulphate has not been determined until now in plasma.
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